We describe a threshold effect of high protein intake and circulating leucine on monocytes/macrophages wherein only protein in excess of ∼25 g per meal induces mTOR activation and functional effects. In a series of clinical studies on male and female participants ( NCT03946774 and NCT03994367) that involved graded amounts of protein ingestion together with detailed plasma amino acid analysis and human monocyte/macrophage experiments, we identify leucine as the key activator of mTOR signalling in macrophages. High protein intake is common in western societies and is often promoted as part of a healthy lifestyle however, amino-acid-mediated mammalian target of rapamycin (mTOR) signalling in macrophages has been implicated in the pathogenesis of ischaemic cardiovascular disease. Nature Metabolism volume 6, pages 359–377 ( 2024) Cite this article J Med Microbiol 46: 314-320.Identification of a leucine-mediated threshold effect governing macrophage mTOR signalling and cardiovascular risk Direct comparison of two commercially available computer programs for analysing DNA fingerprinting gels. Seward RJ, Ehrenstein B, Grundmann HJ, Towner KJ (1997). Two-dimensional gel electrophoretic analysis of vectorially labeled surface proteins of human spermatozoa. Naaby-Hansen S, Flicklinger C, Herr JC (1997). Image-Pro ®®Plus Version 1.0 for Windows ™, Reference Manual, Media Cybernetics Inc. Reproducibility of digital image analysis for measuring corneal haze after myopic photorefractive keratectomy. Maldondo M, Arnau V, Martinez-Costa R, Navea A, Mico F, Cisneros A, Menezo J (1997). A muscle hypertrophy condition in lamb (callipyge): Characterization of effects on muscle growth and meat quality traits. Koohmaraie M, Shackelford SD, Wheeler TL, Lonergan SM, Doumit ME (1995). A simplified method of analysis of cell-conditioned medium for Insulin-like Growth Factor-I (IGF-I) activity. Krabbenhoft EA, O'Reilly BA, Shultz K, Chen Y, Stewart NT, Dodson MV (1997). In: Protein Functionality in Food Systems, New York: Marcel Dekker Inc, pp 79-119. Protein separation and analysis of certain skeletal muscle proteins: Principles and Techniques. Huff-Lonergan EJ, Beekman DD, Parrish Jr FC (1994). Evaluation of satellite cell cultures by computer/video imaging enhancement: An undergraduate research project. Howard JH, Vierck J, Howell S, Dodson MV (1993). Wound status evaluation using color image processing. Hansen G, Sparrow E, Kokate J, Leland K, Iaizzo P (1997). Protein Blotting: A guide to transfer and detection. Collectively, the results of these two studies suggest that specific proteins may be evaluated by using relatively inexpensive image analysis software systems via pixel quantification of electronic images.īio-Rad Laboratories (1996). A second image analysis program, Alpha Imager™ 2000, was then used to define the boundaries of protein bands, assess pixel number and density, and to obtain final numerical data for quantifying α-Actinin on the WB. In the second procedure, WB were scanned with a ScanJet 3c® flat bed scanner and their backgrounds were clarified using Image-Pro® Plus. Dot blots corresponding to a linear concentration range from 10 to 300 ng IGF-Iwere assessed by this method. After the DB were developed and dried, the images were digitized using an HP Deskscan II® flat bed scanner, exported into Image-Pro® Plus and analyzed by taking the combined mean of 45° and 135° sample lines drawn through each dot. In the first procedure, known IGF-I samples were dotted on nitrocellulose membranes using a vacuum manifold. Inexpensive computer imaging technology was used to assess levels of insulin-like growth factor-I (IGF-I) on dot blots (DB) and α-Actinin on Western blots (WB).
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |